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Effect of macrophage migration inhibitory factor (MIF)/tetanus toxoid (TTX) DNA vaccine on elicitation of autoantibodies recognized native MIF protein. Optical density (OD) at 490 nm against anti-MIF antibody in plasma of mice vaccinated with 50 µg of endotoxin-free pCAGGS, wild-type MIF and MIF/TTX in 0·9% sterile saline. Each group consisted of five mice. Results are given as means ± standard error. Statistical significance was assessed compared with pCAGGS-treated mice. *P < 0·05.
