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. Author manuscript; available in PMC: 2010 Dec 28.
Published in final edited form as: J Lipid Res. 2007 Aug 10;48(11):2443–2452. doi: 10.1194/jlr.M700227-JLR200

Fig. 4.

Fig. 4

IL-1β-induced activation of NSMase-2 is not GSH-dependent. A: IL-1β activates endogenous NSMase in the presence of NAC. Hepatocytes from aged (20–22 months) rats were pretreated with 20 mM NAC for 2 h before incubation with 2.5 ng/ml IL-1β for 30 min and subsequent NSMase activity measurements. Results are presented as specific activity of NSMase (hydrolysis of picomoles of substrate per milligram of cellular protein per minute) and are means ± SD (n = 6). B: IL-1β activates exogenous NSMase-2 in the presence of NAC. Hepatocytes from aged (20–22 months) rats were infected with AdNSMase-2, and the expression of NSMase-2 was induced with doxycycline. Cells were treated as described for Fig. 3A, and NSMase activity was measured. Results are presented as specific activity of NSMase (hydrolysis of picomoles of substrate per milligram of cellular protein per minute). Values are means ± SD (n = 6). * P < 0.05, ** P < 0.01.