Figure 3. Electron microscopy images of CysLT₁R and CysLT₂R.

Electron microscopy of Int 407 cells treated without or with (A) LTD₄ (40 nM, 15 or 30 minutes) or (B) LTC₄ (40 nM, 15 or 30 minutes). Samples of intact cells used for electron microscopy were prepared by pelleting 5×10⁶ cells immediately after adding a fixative (4% paraformaldehyde and 0.1% glutaraldehyde). Ultra thin sections were cut on a microtome and mounted on nickel grids, followed by overnight incubation with the primary antibody against CysLT₁R and CysLT₂R. (A) The antibody directed against the CysLT₁R was labeled with 10-nm colloidal thiocyanate gold (black arrow) and CysLT₂R with 5-nm colloidal thiocyanate gold (white arrow). The scale bar represents 0.2 µm. (B) The antibody directed against the CysLT₁R was labeled with 5-nm colloidal thiocyanate gold (white arrow) and CysLT₂R with 10-nm colloidal thiocyanate gold (black arrow). The scale bar represents 0.1 µm. The specimens were examined using a Jeol JEM 1230 electron microscope operated at 60 kV accelerating voltage, and images were recorded with a Gatan Multiscan 791 CCD camera.