Figure 7. AR inhibition prevents RWE-induced expression of IL-13 in mouse lung.

The mice were sensitized and challenged with PBS or RWE, without or with AR inhibitor and 16 h later lungs were harvested and total RNA was isolated (n = 4). One microgram of total RNA from each sample was transcribed into first-strand cDNA and quantitative RT-PCR was conducted using IL-13 specific forward and reverse primers. The levels of RNA for the target sequences were determined by melting curve analysis. The values presented here are fold-change over the control. (^** p<0.001). RWE, ragweed pollen extract; ARI, aldose reductase inhibitor.