Table 1. Yeast two-hybrid analysis for MKS1, MPK4, WRKY25 and WRKY33 proteins.
pBD/pAD | |||||
vector | MKS1ΔN1 | MKS1ΔN2 | MKS1ΔC1 | MKS1ΔC2 | MKS1ΔC3 |
MPK4 | - | N/A | + | + | + |
WRKY25 | + | N/A | + | - | - |
WRKY33 | + | N/A | + | + | - |
Yeast was spotted onto SD-2 medium lacking trp and leu and SD-4 medium also lacking ade and his, and all combinations that grew on both media were scored as an interaction (+).
No interaction is indicated by (-). MKS1ΔN2 was selfactivated.
N/A not applicable.