Figure 2. Western blot analysis of key target proteins of mTOR pathway.

Analysis of total protein and/or phosphorylated forms of (A) mTOR and TSC2, (B) eIF4E, (C) 4EBP1, (D) S6, (E) eEF2 and eEF2K (F) p70S6K, (G) PI3K and PDK1, (H) Akt and (I) ERK1/2, p38 MAPK and PKC. 50 µg of cell lysate was used for analysis of mTOR, TSC2, PI3K, PDK1, Akt, eEF2, 4EBP1 and S6 and 100 µg was used for detection of phosphorylated forms and other proteins.