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. 2010 Dec 28;5(12):e14408. doi: 10.1371/journal.pone.0014408

Figure 4. Analysis of the differential effect of n-Butanol, U0126, Wortmannin and LY294002 on reporter gene expression and phosphorylation of mTOR in transfected PC3 and 293T cells.

Figure 4

Effect of n-butanol on expression of (A) GFP; (B) β-Gal, and (C) Inhibition of phosphorylation of mTOR by n-butanol; Effect of U0126 (U0), Wortmannin (Wort) and LY294002 (LY) on (D) β-Gal and (E) GFP expression. Proteins were separated by 12–14% SDS-PAGE and detected by immunoblotting using antibodies specific to GFP and β-Gal.