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. 2010 Dec 28;5(12):e14434. doi: 10.1371/journal.pone.0014434

Figure 1. Generation of LARGE transgenic mouse.

Figure 1

A. A schematic representation of the LARGE expression cassette. The full-length V5 tagged LARGE cDNA was subcloned into the pCAGGS expression vector. This vector consists of a synthetic CMVenhancer/and chicken β-actin promoter sequence located upstream of the transgene and the rabbit β-globin poly(A) sequence located downstream. Arrows represent position and orientation of PCR primers used to screen ear biopsies. B. Western blotting analysis of protein lysates from wild type and muscle tissues (quadriceps) from all the four LARGE transgenic lines (68,76,116 and 126) using antibodies to α-DG IIH6, β-DG, and V5.