Figure 6. The ectopic expression of BRCA1 reverts the generation of aberrant centrosomes induced by BCR/ABL.

a) Analysis of MIN-R1 or MIN-210-transduced cord blood CD34⁺cells with supernumerary centrosomes after re-infection with either Neor or BRCA1/Neor RVs. In all instances cells were exposed to 40 nM MMC prior to analysis. Data corresponding to one representative experiment is shown. b) Representative pictures corresponding to panel a) showing supernumerary centrosomes in MIN-210 Neo^r compared to MIN-R1 Neo^r cells. To identify centrosomes γ-tubulin antibody (green) was used. DAPI staining is shown in blue.