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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Leuk Res. 2010 Sep 25;35(3):305–309. doi: 10.1016/j.leukres.2010.08.011

Figure 2. Knockdown of AF4-MLL has no effect on cell growth.

Figure 2

Leukemia cell lines SEMK2-M1, RS4;11 and HPB-NULL were electroporated with various siRNAs as described and viable cells were counted by Trypan blue exclusion. Daily cell counts were normalized to those on day 4 which were considered 100%. Graphs below depict growth curves for each cell line with time since electroporation indicated on the x-axis. Data represent mean ± std. err. of 3 separate experiments. In the SEMK2-M1 repeat electroporation experiment, SEMK2-M1 cells were electroporated sequentially on days 0, 2, 4 and 7 (indicated by arrows on the x-axis) observed for a total of 9 days. Viable cells were counted on the days of electroporation and normalized to counts on day 9 (100%).