Table 1.
Real Time PCR Primers
| TARGET | Real Time PCR Forward Primer Sequence (5’-3’) |
Real Time PCR Reverse Primer Sequence (5’-3’) |
Base Pair Size of Amplicon (bp) |
|---|---|---|---|
| rPDE4A | gcctgatcctttttcccttc | tttggggacttcagttcctg | 221 |
| rPDE4B | cccgcagctgtagtagaagc | caggcatttcaaagacacga | 215 |
| rPDE4C | gcagcatggttcaatggtta | atatcagctgggagcctggt | 159 |
| rPDE4D | agaggaagatggcgagtcag | tcaggacaacaatcgtctgc | 201 |
| rPDE4B1 | gcaaacagcagacatctcca | ccttgccctttcactttgtc | 236 |
| rPDE4B2 | gtcttgtccgggtcagtgtt | gctttcccctctctttgctt | 165 |
| rPDE4B3 | gccaggctttgcttactgtc | gccaaagtgcttcctctgtc | 209 |
| rPDE4B4 | cctcgtgtgtcgattgctaa | tctccatgcgtttctctgtg | 150 |
| rPDE4B5 | tctgtatcttggggttacatca | gttacgaaggtgtcggagga | ~445 |
| β-actin | actcttccagccttccttc | atctccttctgcatcctgtc | 171 |