Table 1. Data Collection and Refinement Statistics.

Data Collection	Kv7.4 (610–645)	Kv7.4 (610–640)
Space group	P 4212	I4
Cell dimensions
a, b, c (Å)	30.53, 30.53, 139.94	33.18, 33.18, 55.28
α, β, γ (°)	90, 90, 90	90, 90, 90
Resolution (Å)	50–2.10 (2.18–2.10)	50–2.07 (2.14–2.07)
Rsym	11.3 (52.7)	5.3 (9.6)
I/σI	18.7 (2.5)	28.4 (12.6)
Completeness (%)	96.7 (89.5)	99.6 (100.0)
Redundancy	12.5 (7.2)	7.3 (6.1)
Refinement
Resolution (Å)	2.10	2.07
No. reflections	53,620	13,350
Rwork/Rfree	32.1 / 36.1	19.6 / 22.4
Total protein atoms		249
Water molecules		18
Average B factors: protein (Å2)		21.1
Average B factors: water (Å2)		36.5
RMSD in bond lengths (Å)		0.014
RMSD in bond angles (°)		1.59

Values in parentheses are for the highest-resolution shell.

$${\text{R}}_{\text{sym}}=\sum _{\text{hkl}}\sum _{\text{i}}[|{\text{I}}_{\tau }(\text{hkl})-<\text{I}(\text{hkl})>|/{\text{I}}_{\text{i}}(\text{hkl})]$$

$$R_{\text{work}}=\sum _{\text{hkl}}\Vert {\text{F}}_{\text{obs}}(\text{hkl})|-|{\text{F}}_{\text{calc}}(\text{hkl})\Vert /\sum _{\text{hkl}}\left|{\text{F}}_{\text{obs}}(\text{hkl})\right|$$

R_free = R_work calculated using 5% of the reflection data chosen randomly and omitted from the start of refinement.