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. 2010 Oct-Dec;4(4):491–501. doi: 10.4161/cam.4.4.12661

Figure 2.

Figure 2

Study of E-cadherin dynamics using photoactivation. (A) Representative images captured pre-activation and following activation showing basal fluorescence of PAGFP-E-cadherin and PAGFP-Farn2Palm when illuminated with 488 laser. Fluorescence intensity heat maps have been applied to facilitate visualization. Scale bars, 20 µm. (B) Representative still images of PAGFP-E-cadherin (top parts) and PAGFP-Farn2Palm (bottom parts) at the plasma membrane captured pre-activation and following activation. Fluorescence intensity heat maps have been applied to facilitate visualization. Scale bars, 5 µm. (C) Fluorescence decay curves following photoactivation of PAGFP-E-cadherin (red) and PAGFP-Farn2Palm (blue). (D) Quantification of t1/2 following photoactivation of PAGFP-E-cadherin and PAGFP-Farn2Palm. Values represent the mean from at least 25 cells. Error bars, s.e.m.