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. 2010 Oct-Dec;4(4):491–501. doi: 10.4161/cam.4.4.12661

Figure 3.

Figure 3

Monitoring E-cadherin dynamics at the plasma membrane. (A) Integrated fluorescence intensity measurements over the plasma membrane at activation (full line) and t1/2 time points (dash line) were fitted to Gaussian curves for PAGFP-E-cadherin (top) and PAGFP-Farn2Palm (bottom). (B) Quantification of the speed of lateral movement of PAGFP-E-cadherin and PAGFP-Farn2Palm within the membrane. (C) Integrated fluorescence intensity measurements over the plasma membrane at bleaching (full line) and t1/2 time points (dash line) were fitted to Gaussian curves for GFP-E-cadherin (top) and GFP-Farn2Palm (bottom). (D) Quantification of the speed of lateral movement of GFP-E-cadherin and GFP-Farn2Palm within the membrane. Values represent the mean from at least 25 cells. Error bars, s.e.m.