Figure 7.

Inhibition of endocytosis strengthens cell-cell adhesion. (A) Representative images of fragments detached from a dispase-treated monolayer of control or dynasore (80 µM, 30 min) pre-treated A431 cells after mechanical stress. (B) Quantification of the number of single cells that disaggregate from a dispase-treated monolayer after mechanical stress. (C) Quantification of the number of single cells that disaggregate after mechanical stress from a dispase-treated monolayer of A431 or A431 GFP-E-cadherin cells, transfected with either control or E-cadherin siRNA. (D) Still images obtained from photobleaching experiments showing GFP-E-cadherin localization in A431 cells untreated or treated with dynasore (80 µM, 30 min). Scale bars, 20 µm. (E) Immunoblot showing surface and total levels of GFP- and endogenous E-cadherin (150 kDa and 120 kDa, respectively) in A431 cells untreated or treated with dynasore (80 µM, 30 min). (B and C) Values represent the mean from at least three independent experiments. Error bars, s.e.m.