. Author manuscript; available in PMC: 2010 Dec 29.

Published in final edited form as: Technol Cancer Res Treat. 2010 Apr;9(2):139–147. doi: 10.1177/153303461000900203

Table III.

Summary of next-generation sequencing technologies.

	Template preparation	Synthesis method	Read length (bases)	Run time	Gb per run
Roche/454 GS FLX Titanium	Emulsion PCR	Pyrosequencing	Average 400	10 hr	0.4
Illumina/Solexa Genome Analyzer IIx	Bridge PCR	Reversible terminators	Up to 100	4^, 9.5^* days	17^, 33^*
Life/SOLiD 3	Emulsion PCR	Ligation	Up to 50	7^, 14^* days	30^, 60^*

Single-end read sequencing;

^**

Paired-end read Sequencing.