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Low oxygen and the presence of UBM digest material increase phosphorylation of ERK1/2. The phosphorylation of ERK1/2 after exposure of perivascular stem cells to 0, 50, or 100 μg/mL UBM digest material for 16 h at 6% or 21% oxygen was monitored via immunoblot. The equivalent intensity of the bands for β-actin and ERK across loading conditions indicates equal loading of protein per well.
