Table 2.
Flow Cytometric Characterization of Small CD90+, Large CD90+ and CD90− Nonhematopoietic Breast Cancer Cells Isolated from Pleural Effusions
| |
|
|
Nonheme CD45−/Lin− |
||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| |
|
|
CD90+ |
|
|
|
|||||
| |
|
|
High light scatter CD90+ |
Low light scatter CD90+ |
CD90− |
||||||
| Mean (%) | SD (%) | SEM (%) | Mean (%) | SD (%) | SEM (%) | Mean (%) | SD (%) | SEM (%) | |||
| Cell cycle | G0/G1 | DNA = 2N | 89.44 | 13.75 | 5.20 | 97.88 | 1.61 | 0.61 | 94.12 | 3.96 | 1.50 |
| Cycling or aneuploid | DNA > 2N | 10.56 | 2.12 | 5.88 | |||||||
| Expressing | Epithelial markers | Pan cytokeratin | 88.59 | 15.13 | 4.78 | 44.38 | 36.25 | 11.46 | 40.97 | 37.56 | 11.88 |
| Epithelial cell adhesion molecule | 10.83 | 20.89 | 7.38 | 1.79 | 3.11 | 1.10 | 23.49 | 33.00 | 11.67 | ||
| Stem cell markers | CD44 | 84.68 | 9.26 | 4.14 | 30.71 | 23.62 | 10.56 | 40.87 | 42.11 | 18.83 | |
| CD117 | 1.68 | 2.33 | 0.78 | 2.06 | 4.80 | 1.60 | 1.69 | 2.35 | 0.78 | ||
| CD133 | 4.86 | 5.18 | 1.83 | 0.63 | 1.25 | 0.44 | 4.11 | 5.18 | 1.83 | ||
SD, standard deviation; SEM, standard error of the mean.