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. 2010 Dec 6;17(1-2):139–150. doi: 10.1089/ten.tea.2010.0199

FIG. 4.

FIG. 4.

Gene expression analysis. RLE-6TN cells were cultured for 48 h on Fn fragments (FnIII9’10 and FnIII10), and then RNA was isolated for gene expression analysis. Levels of expression of epithelial (E-cad, ZO-1) and mesenchymal (α-smooth muscle actin [SMA], Col I, Vimentin, and N-cad) markers were determined by quantitative polymerase chain reaction. Fold changes in gene expression were determined by ΔΔCT analysis using glyceraldehyde 3-phosphate dehydrogenase as the endogenous control and comparing expression to cells cultured on FnIII9’10. Reactions were performed in triplicate. ***p < 0.001, *p < 0.05.