FIG. 5.

E-cadherin and α-SMA staining in RLE-6TN cells cultured on Fn fragments. RLE-6TN cells were cultured on FnIII9’10 (A, C, E, G) or FnIII10 (B, D, F, H) in the absence (A, B, E, F) or presence (C, D, G, H) of 5 ng/mL active transforming growth factor-β (TGFβ) for 48 h. Cells were then analyzed for E-cadherin (A–D) and α-SMA (E–H) expression and distribution via immunofluorescence staining. Fluorescent images were acquired with a Nikon Eclipse (TiE) inverted fluorescence microscope at 20× magnification. Images are of representative of at least three independent experiments. Color images available online at www.liebertonline.com/ten.