FIG. 7.

Plasminogen activator inhibitor-1 gene expression analysis. RLE-6TN cells were cultured for 48 h on FnIII9’10, FnIII10 (A), Fn, or Ln (B) in the absence or presence of 5 ng/mL active TGFβ. Levels of expression of the TGFβ responsive gene plasminogen activator inhibitor-1 were determined by quantitative polymerase chain reaction. Fold changes in gene expression were determined by ΔΔCT analysis using glyceraldehyde 3-phosphate dehydrogenase as the endogenous control and comparing expression to cells cultured on FnIII9’10. Reactions were performed in triplicate. ***p < 0.001, *p < 0.05.