FIG. 8.

Wound healing responses on Fn fragments. A wound healing assay was performed with RLE-6TN cells plated on FnIII9’10 (A, C), FnIII10 (B, D) in the absence (A, B) or presence (C, D) of 5 ng/mL active TGFβ. Controls included cells plated on Fn (E) and Ln (F). Cells were allowed to form a monolayer around a wound field insert, which was removed 5 h post-seeding. Cells were allowed to migrate and close the wound field for 15 h and then fixed, and their nuclei stained. Cells were imaged at 10× magnification. Percent wound closure was determined (G) by measuring the wound gap area and dividing by original wound area and multiplying by 100%. At least three images were taken per condition, and conditions were run in triplicate. ***p < 0.001, *p < 0.05.