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. 2010 Aug 25;17(1):61–67. doi: 10.1089/ten.tec.2010.0305

FIG. 4.

FIG. 4.

Co-culture arrays on Transwell. The surface of the membrane was treated with (A, B) or without (C, D) fibronectin. COS7 cells were labeled with CellTracker green and HepG2 cells were labeled with CellTracker red. (A) Cells spread out over the PDMS pattered surface, and thus the cellular patterns disappeared on day 4. (B) COS7 cells were seeded 1 day after HepG2 cells had been patterned. (C, D) COS7 cells gradually spread out over the PDMS patterned surface without fibronectin coating after 4 days in culture, whereas HepG2 cells do not spread into the PDMS regions. (D) COS7 cells were seeded 3 days after HepG2 cells had been patterned. Scale bars: 300 μm. Color images available online at www.liebertonline.com/ten.