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. 2010 Aug 25;17(1):61–67. doi: 10.1089/ten.tec.2010.0305

FIG. 6.

FIG. 6.

Evaluation of mES cell differentiation. (A) Schematic illustration of experimental conditions. mES cells stably transfected with SOX17-EGFP were cultured in the top chamber for 3 days to form semi-spherical aggregates. HepG2 cells were then introduced into either the bottom chamber (Co-culture A, above-and-below mode) or the top chamber (Co-culture B, side-by-side mode) on day 3 and they were co-cultured for 4 days. (B) Relative EGFP intensity of mES cells. mES cells were cultured without (mono-culture) or with HepG2 cells for 7 days. Values represent the mean ± standard deviation. All three conditions are significantly different. p < 0.0001. Color images available online at www.liebertonline.com/ten.