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. Author manuscript; available in PMC: 2011 Dec 10.
Published in final edited form as: Circ Res. 2010 Oct 14;107(12):1470–1482. doi: 10.1161/CIRCRESAHA.110.227371

Figure 6. FoxO1 regulates Rab7 expression and FoxO1-induced autophagy is attenuated by Rab7 knockdown.

Figure 6

Figure 6

A) CMs were transduced with Ad-LacZ, Ad-FoxO1-WT, Ad-3A/LXXAA or Ad-sh-FoxO1. Immunoblots and densitometric analyses showing Rab7 expression. B) CMs were transduced with Ad-sh-FoxO1 or Ad-sh-Scr and treated with glucose free media. Immunoblot and densitometric analyses showing Rab7 expression. C) CMs were transduced with Ad-HA-Rab7 or Ad-LacZ. Immunoblots and densitometric analyses. D) CMs were transduced with Ad-LacZ, Ad-FoxO1-WT, Ad-sh-Scr or lentivirus harboring sh-Rab7 (Lt-sh-Rab7) for 96 hours. Immunoblots for LC3 (at two different exposure times) and p62 and densitometric analyses are shown. Data represent means from at least 4 independent experiments. * p<0.05, ** p<0.01.