FIG. 4.
Fitness of CP and VC virus and viral constructs with sequences of relevant gag regions displayed below each experiment. (A) Fitness of recombinant pseudotyped NL43 constructs in a single cycle assay. Results depict duplicate or triplicate experiments in one donor. (B) Fitness of replication-competent virus from VC and CP as compared to Ba-L laboratory strain virus, as measured by p24 ELISA. Results depict triplicate experiments. (C) Fitness of CP and VC gag-NL43 constructs containing substitutions in residues 94 and/or 95. Results depict duplicate or triplicate experiments in three different donors. (D) Fitness of replication-competent recombinant gag-NL43 mutants. Results depict duplicate or triplicate experiments in three different donors. (Color image can be found at www.liebertonline.com/aid).