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. 2010 Nov 11;2:888–896. doi: 10.1093/gbe/evq073

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© The Author(s) 2010. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution.

This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/2.5), which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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FIG. 2.— — Yeast two-hybrid analysis based on the activation of the reporter gene HIS. Different combinations of bait and prey proteins are indicated above the growth plates. FNR/220, CSK/pBD, and PTK/pBD are negative controls. Test combination of bait and prey proteins are CSK/CSK, CSK/SIG-1, CSK/TCP34, PTK/CSK, PTK/SIG-1, and PTK/TCP34. The combination FNR/IA2 is the positive control. (A) Yeast cells growing on synthetic SD media plates lacking leucine and tryptophan. The growth of all bait and prey combinations in this medium confirms successful transformation of yeast cells with both bait and prey plasmids. (B) Growth of yeast cells in SD media plates without leucine, tryptophan, and histidine. The growth in –His plates require the activation of the histidine biosynthetic gene (His reporter gene), which in turn requires the functional interaction between bait and prey proteins.