Table 1.
Overview of the observed phenotypes of EScaAG VIGS in California poppy
| Phenotypes observed | pTRV1/pTRV2-E | pTRV1/pTRV2-EScaAG1 | pTRV1/pTRV2-EScaAG2 | pTRV1/pTRV2-EScaAG1+2 | |
|---|---|---|---|---|---|
| 1 | No. of inoculated plants | 12 | 120 | 120 | 120 |
| 2 | No. of analyzed flowers | 36 | 239 | 209 | 261 |
| 3 | No. of flowers showing phenotype in the third and fourth whorls | 0 | 122 (51.0%) | 118 (56.4%) | 174(66.6%) |
| 3.1 | No. of flowers with homeotic conversions in the stamens | 0 | 67 (54.9%) | 53 (44.9%) | 113 (64.9%) |
| 3.1.1 | No. of flowers with transformation of all the stamens into petals | 0 | 3 (4.4%) | 8 (15%) | 17 (15%) |
| 3.1.2 | No. of flowers showing only outer stamen whorls converted into petaloid organs | 0 | 64 (95.5%) | 0 | 0 |
| 3.1.3 | No. of flowers showing only inner stamen whorls converted into petaloid organs | 0 | 0 | 45 (84.9%) | 0 |
| 3.1.4 | No. of flowers showing only outer and inner stamen whorls converted into petaloid organs | 0 | 0 | 0 | 96 (84.9%) |
| 3.2 | No. of flowers with alterations in the carpels | 0 | 27 (22.1%) | 31 (26.2%) | 40 (22.9%) |
| 3.2.1 | No. of flowers with flattened green gynoecium | 0 | 23 (85.1%) | 26 (83.8%) | 32 (80%) |
| 3.2.2 | No. of flowers with an orange pigmented gynoecium | 0 | 4 (17.3%) | 5 (16.1%) | 8 (20%) |
| 3.3 | No. of flowers showing defects in the floral meristem termination | 0 | 62 (50.8%) | 80 (67.7%) | 110 (63.2%) |