Figure 3.

Downregulation of Pim-1 inhibited the bladder cells growth and sensitized them to Doxorubicin and Docetaxel treatment. A. Knockdown of Pim-1 decreased the phosphorylation of Bad and the expression of Bcl-2. The cells were infected lentivirus siRNA specific for Pim-1(si Pim-1) or vector control. At 48 h postinfection, cells were lysed and the lysates were subjected to western blot with indicated antibody. B. Downregulation of Pim-1 inhibited the bladder cancer cell growth. Total of 1 × 10⁴ T24 and UM-UC-3 cells were plated in each well of a 6-well plate and infected with lentivirus encoding Pim-1 siRNA or vector control siRNA. The cell culture was maintained in complete medium for two weeks. Finally, the cell colonies were visualized by Coomassie blue staining. C. Decreased expression of Pim-1 sensitized bladder cancer cells to Doxorubicin and Docetaxel treatment. The cells were plated on 96 wells and infected with lentivirus encoding Pim-1 siRNA or vector control siRNA. At postinfection for 48 h, cells were treated with DOX (T24, 2.5 and 5μg/ml; UM-UC-3, 1.25 and 2.5 μg/ml) and DTX (T24, 25 and 50 nm; UM-UC-3, 2.5 and 5 nm) for another 48 h. The cell viability was assessed by WST-1 assay.*, p < 0.05 compared with the control; **, p < 0.01 compared with control.