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External calcium was varied while extracellular magnesium concentration remained constant at 1.5 mM. EPSC peak amplitudes measured in 1 mM and 10 mM were normalized to the values measured in 2.5 mM calcium. miR132 overexpression had no significant effect on the calcium dependence of release (for all groups, n = 9, t-test for 1 mM and 10 mM P>0.05; scale bar for representative traces: 1nA, 10 ms).
