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. 2010 Dec 29;5(12):e14452. doi: 10.1371/journal.pone.0014452

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Tapirdamaz et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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CFSE-labeled PBMC from 5 LTx-patients were stimulated with donor-derived or 3^rd party CD40-B cells, and re-stimulated with the same allo-antigens at day 5 of culture for 24 hours. During the last 15 hours Brefeldin A was added, and CFSE-dilution and intracellular IFN-γ was determined at day 6. Depicted are the percentages of T cells producing IFN-γ in response to donor-derived CD40-B cells and 3^rd party-derived CD40-B cells, and the RR of IFN-γ producing T cells. No statistically significant differences were observed in comparing RR at 1 year after LTx versus before LTx (p≥0.44).