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. 2010 Dec 29;5(12):e15741. doi: 10.1371/journal.pone.0015741

Table 1. Genotyping Table.

Clone
Embryo number 5A5 5C7 5D6 5G1 5H8 5I7 6E6 6F7 7D7 5I2 Nb of different clones integrated
59 x x x x x 5
60 x x 2
61 x x x x x x x 7
62 x x 2
63 x 1
64 x 1
65 0
66 x x x 3
67 0
68 x x x x x x 6
69 x 1
70 x x 2
71 x x 2
72 x x x x 4
91 x x x 3
92 x x x 3
93 x x x x x x x 7
94 x x x x 4
177 x x x x 4
178 x x 2
179 x x 2
180 x x x x x x x 7
181 x x x 3
182 0
183 x x 2
184 x x 2
185 x x x 3
186 x x x x x 5
187 x x x x x x x 7
188 0
189 x 1
190 x x x x x x 6
191 x x x x 4
192 x x 2
193 x x x x x x 6
194 x x 2
195 0
196 x x x 3
197 x x x x 4
198 x x x x x 5
199 x x 2
200 x x x x 4
201 x x x 3
20 20 12 10 21 0 0 21 25 0

Example of a genotyping table. Pool B clones are displayed horizontally and embryo numbers vertically. X indicates that the embryo integrated the corresponding clone. Embryo numbers in bold face indicate LacZ positive animals. Candidate clones were identified by first looking for a repetition of a specific staining pattern among embryos injected with a pool of sequences. Within the subset of embryos that displayed the same pattern, we looked for common integrated clones. These fragments were the candidate enhancers to be injected individually for confirmation.