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A. Deletion map of ΔasdA01; B. Genotype verification of J112 ΔasdA01 by PCR; C. Phenotype of E. ictaluri J111 ΔasdA01 and J112 ΔasdA01 mutants. The strains were grown in BHI at 28°C with agitation (180 r.p.m.).
