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Immunofluorescence analysis of cGKI and glucagon. Colocalization (white arrows) of glucagon (green) and cGKI (red) was detectable only in islets of control mice (merge). The glucagon signal in cGKI-KO (KO) and αRM animals was well detectable in the mantle of the islets, but no coexpression with the cGKI protein was found. Scale bar, 20 μm. (A high-quality color representation of this figure is available in the online issue.)
