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. 2010 Oct 7;60(1):157–167. doi: 10.2337/db10-0331

FIG. 4.

FIG. 4.

Changes in PGC-1α expression and acetylation after chronic exercise. A: Real-time PCR analyses of PGC-1α mRNA expression in the skeletal muscle of C57BL/6J wild-type mice (black columns) and ob/ob mice (gray columns). All data are normalized per 18S rRNA. B: Densitometry of protein data. Homogenates of skeletal muscle were probed with an antibody detecting PGC-1α (90 kDa). Blots were also probed with GAPDH as a loading control. C: Densitometry of immunoprecipitation (IP) experiments performed on skeletal muscle lysates, using PGC-1α for precipitation and an antibody directed against acetyl-lysine or PGC-1α for detection. Data are given as acetylated PGC-1α per total PGC-1α in these samples. All data are means ± SEM. *P < 0.05; **P < 0.01. C, controls; r.U., relative units; T, trained mice. D: Representative full-size blots of IP experiments.