FIG. 1.

Toxicity of saturated NEFAs according to chain length (C10:0-C18:0) in RINm5F insulin-producing cells. Cells were incubated for 24 h with saturated NEFAs of different chain lengths and viability was determined by MTT assay (A) or propidium iodide staining (B). EC₅₀ values were calculated by nonlinear regression analysis. Data are means ± SEM from 4–6 individual experiments. *P < 0.01 as compared with C10:0, C11:0, C12:0, or C13:0. Other comparisons were not significant (ANOVA/Tukey test for multiple comparisons).