FIG. 1.
Comparison of expression of Src between fresh Wistar and GK islets. Fresh islets were lysated and subjected to immunoblot analyses. Blots (50 μg of protein) were probed with anti–phospho-Src (Tyr416), anti–phospho-Src (Tyr527), anti-Src, or anti-Csk. The same blots were stripped and reprobed with anti–β-actin, respectively. Intensities of the bands were quantified with densitometric imager. The bar graphs are expressed relative to Wistar islet value corrected by β-actin level (means ± SE). *P < 0.05; †P < 0.01; ‡P < 0.001. Representative blot panels of three to five independent experiments are shown.