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. 2010 Oct 13;60(1):258–268. doi: 10.2337/db10-0281

FIG. 2.

FIG. 2.

Effect of FTO overexpression on insulin signaling in HEK293 cells. HEK293 were transiently transfected with pcDNA3-FTO or empty pcDNA3 vector (control). Forty-eight h posttransfection, cells were depleted in serum for 3 h and stimulated with insulin (10−7 M, 10 min). A: Representative Western blots of FTO, pSer473PKB, pThr308PKB, and total PKB. B: Histogram illustrates the quantification and normalization of the phosphorylation of PKB in control and FTO-overexpressing cells. Values are means ± SEM (n = 3). *P < 0.001 versus control cells, #P < 0.001 FTO versus GFP. Ins, insulin.