FIG. 5.

Real-time qPCR data for murine hypothalamic genes whose regulation by hypoglycemia were not impaired by antecedent hypoglycemia but were prevented by naloxone, though not by valsartan. Relative expression levels of (A) Gpd2 (glycerol 3-phosphate dehydrogenase 2, mitochondrial), (B) Cxcl14, and (C) Sox17 were assessed using custom PCR arrays from SABiosciences in the same groups described in Fig. 1. Animals were killed 4 h after the final insulin or saline injection. Data for each gene were normalized to a panel of housekeeping transcripts and expressed as fold change compared with the saline-injected (euglycemic) group. Data are means ± SE (n = 6 for all groups). *P < 0.05 as compared with the euglycemic group (Dunnett test).