FIG. 2.

A: Representative hematoxylin- and eosin-stained histological sections of endogenous epididymal, endogenous mesenteric, or portal-transplanted fat pads from sham-operated and pTx mice are shown 5 weeks after surgery. Adipocyte cell perimeter was measured using ImageJ. B: Up to 100 cells for each fat pad were analyzed, and the results represent the mean ± SEM of three or four different mice. Black bar: sham-operated mice; white bar: mice receiving a portal fat transplant. C: Histological sections of endogenous epididymal and portal-transplanted tissue were immunostained with mac2 antibody. Mac2-positive cells were counted in seven randomly selected fields per section (about 90 cells) and expressed relative to total cell nucleus content. Results represent the mean ± SEM of three to five different mice. All values are expressed relative to Mac2-positive cells of epididymal fat pad of sham-operated mice.