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. 2001 Feb 20;98(5):2238–2243. doi: 10.1073/pnas.041597098

Figure 2.

Figure 2

Expression of Tm-Cyto inhibits the N-CAM-induced NF-κB activation in transfected primary wild-type astrocytes but not in N-CAM-null cells. (A) Immunoblot of astrocytes transfected with pcDNA3.1+ (lane 1), pcDNA-Tm-Cyto (lane 2), or pcDNA-Tm-Cyto/palm− (lane 3). Upper bands (140 kDa) are endogenous sd N-CAM; lower bands are the Tm-Cyto proteins. (B) The transfected cells were treated and assayed as described in Materials and Methods. The results are shown as the ratio of the luciferase activity normalized to the β-galactosidase activity for each treatment condition. The error bars represent the standard deviation from quadruplicate samples of a representative experiment. Each experiment was replicated a minimum of four times. (C) Immunoblot of N-CAM knockout astrocytes transfected with pcDNA3.1+ (lane 1) or pcDNA-Tm-Cyto (lane 2). (D) Luciferase assay in transfected knock-out astrocytes. The results are shown as described above.