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. 2001 Feb 20;98(5):2238–2243. doi: 10.1073/pnas.041597098

Figure 4.

Figure 4

IgIII-induced NF-κB activation is inhibited by the expression of Tm-Cyto truncation mutants containing the amino acids 780–790. (A) Immunoblot of astrocytes transfected with Tm-Cyto constructs. Lane 1, pcDNA3.1+; lane 2, Tm-Cyto; lane 3, Tm-CytoΔ824–839; lane 4, Tm-CytoΔ801–839; lane 5, Tm-CytoΔ791–839; lane 6, Tm-CytoΔ780–839; lane 7, Tm-CytoΔ745–839; and lane 8, GFP-Δ745–839. The band at higher molecular weight in lane 8 is nonspecific and is present in nontransfected astrocytes (data not shown). (B) Luciferase activity in transfected astrocytes. The results are shown as described above.