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Specific threonine to alanine or aspartate mutations in the Tm-Cyto construct block IgIII-induced NF-κB activation. (A) Immunoblots of transfected astrocytes. Lane 1, pcDNA3.1+ ; lane 2, Tm-Cyto; lane 3, Tm-Cyto T788A; lane 4, Tm-Cyto T793A; lane 5, Tm-Cyto T794A; lane 6, Tm-Cyto T797A; lane 7, Tm-Cyto T788D; lane 8, Tm-Cyto T794D; and lane 9, Tm-Cyto T797D. (B) Luciferase activity of transfected astrocytes. The results are shown as described above.
