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. Author manuscript; available in PMC: 2010 Dec 30.
Published in final edited form as: Methods Enzymol. 2010;478:343–363. doi: 10.1016/S0076-6879(10)78017-4

Figure 4. Siglec-Fc beads enable binding at elevated temperature without endocytosis to reveal improved binding.

Figure 4

Human CD22-Fc beads were probed with high molecular weight LacNAc-PAA or BPCNeuAcα2,6-LacNAc-PAA at 4 °C or 37 °C for 16 hours.