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. Author manuscript; available in PMC: 2010 Dec 30.
Published in final edited form as: Methods Enzymol. 2010;478:343–363. doi: 10.1016/S0076-6879(10)78017-4

Figure 6. Tuning the density of biotinylated ligand-coated beads to modulate avidity of siglec-Fc binding.

Figure 6

Streptavidin beads were coated with biotinylated NeuAcα2,8-NeuAcα2,3-Galβ1,4-GlcNAc after pre-treatment of beads with varying concentrations of free biotin to adjust the density of carbohydrate ligands. Beads were then probed with siglec-E-Fc from culture supernatant in the presence of the detection antibody, FITC-anti-human IgG. Control beads had neither biotin nor ligand.