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. 2011 Jan;162(1):111–125. doi: 10.1111/j.1476-5381.2010.01032.x

Figure 8.

Figure 8

Effects of peroxisome proliferator-activated receptor (PPAR) agonists on the expression of nephrin and synaptopodin. Cells were treated with gemfibrozil (10 µM), GW0742 (0.1 µM), pioglitazone (1 µM) or rosiglitazone (0.1 µM) following Protocol 3 and exposed to serum deprivation (SD; 48 h). (A) Nephrin and synaptopodin expression was evaluated at both mRNA (A) and protein level (B) by real-time PCR (A), Western blot (B) respectively. (C) Representative micrographs of nephrin and synaptopodin expression detected by immunofluorescence in podocytes cultured with serum (control) exposed to serum deprivation (SD) and treated with rosiglitazone (original magnification: 630×). Results are expressed as mean ± SEmean of four experiments run in triplicate. **P < 0.01 versus vehicle alone; °P < 0.01 versus basal.