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. Author manuscript; available in PMC: 2010 Dec 30.
Published in final edited form as: Annu Rev Psychol. 2010;61:111–C3. doi: 10.1146/annurev.psych.093008.100359

Figure 4.

Figure 4

Methods for studies of adult neurogenesis are not equally sensitive. (A) BrdU antibodies do not label the same number of newborn cells in the dentate gyrus. Vector and Novocastra antibodies stain fewer BrdU-labeled cells as compared to BD, Roche, Dako, and Accurate antibodies (two-hour post-BrdU survival time). (B) Additional variability in BrdU labeling occurs with different DNA denaturation pretreatment methods. HCl alone and HCl + formamide pretreatments stain more newborn cells in the dentate gyrus than does steam heating. (C) Pretreatments also differentially affect immunoflurorescent staining for the mature neuronal marker, NeuN; greater staining is observed with HCl-alone pretreatment. *p < 0.05. Adapted from Leuner et al. (2009).