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. Author manuscript; available in PMC: 2012 Mar 1.
Published in final edited form as: Cell Signal. 2010 Nov 29;23(3):594–601. doi: 10.1016/j.cellsig.2010.11.013

Fig. 10.

Fig. 10

Profiling the specificity of BC76 using the 5FOA growth assay. Strains expressing 15 of the 21 mammalian PDE genes were tested for growth stimulation by BC76 in the 5FOA growth assay. A) Data for strains that show growth stimulation in response to BC76. B) Data for strains that show no response to BC76. Values in panels A and B represent the average OD600 from duplicate wells after 48hr growth at 30°C. C) Conditions used for the 15 strains, including the pregrowth supplement to repress fbp1-ura4 transcription prior to inoculation of cells into 5FOA medium, the amount of cAMP or cGMP present in the 5FOA medium (NA= not applicable as the strain used can synthesise cAMP), and the initial cell density for a given strain.