Fig. 8.
Exogenous cGMP and BC76 synergistically activate PKA in a PDE5A-expressing strain to alter cell length. Exponential phase cells of strain CHP1223 (git2Δ cgs2Δ::PDE5A) growing in EMM medium containing no cGMP (panels A and D), 0.5mM cGMP (panels B and E), or 1.0mM cGMP (panels C and F) were exposed to DMSO (vehicle control; panels A-C) or 30µM BC76 (panels D-F). Cells were imaged as in Fig. 7 after 4 hours growth at 30°C.