Fig. 3.
Antigen-bearing DCs inhibit TCM development. (A) Naïve 2C cells were injected into CD11c-DTR/EGFP transgenic mice followed by i.n. infection with the WSN-SIY virus. The mice were injected with diphtheria toxin (DT) or PBS 5 dpi. Two days later (7 dpi), 2C cells (2 × 104) from the spleen were transferred into B6 recipient mice and parked for 23 d. Recipient mice then were challenged i.n. with WSN-SIY virus, and 2C cell numbers were counted in BAL fluid 7 d later to measure recall responses (y axis). (B) B6 mice were injected with naive 2C cells and infected with WSN-SIY virus. Activated 2C cells (5 × 105) from DLN 5 dpi were transferred into naive mice or mice that were injected i.v. with BMDCs (1 × 106) loaded with the SIY or control (OVA) peptide upon 2C cell transfer. Twenty-five days posttransfer, recall responses were measured as in A. (C) B6 mice were injected with naive 2C cells and infected with WSN-SIY virus. Activated 2C cells (5 × 103) from NDLN 7 dpi were transferred into mice that also were given SIY peptide or PBS daily for 4 d. The recall response of 2C cells was measured 23 d posttransfer, as in A. Error bars represent the SEM from three recipient mice per group. *P < 0.05; **P < 0.01.